About us
UVIC Genome BC Proteomics Centre, UVIC
PhD
David Goodlett
Dr. Goodlett received his Ph.D. (1991) from North Carolina State University in protein chemistry and mass spectrometry. He moved to Washington state to complete his postdoctoral fellowship at Battelle Memorial Institute (now PNNL) with Dr. Richard D. Smith. He is now a professor in the department of Biochemistry & Microbiology at the University of Victoria where he is also Director of the Genome BC Proteome Centre.
Why do you study metabolism / proteomics?
I did my PhD in protein chemistry when MS was just beginning to be useful and this passion I had for protein structure grew into proteomics and later microbial lipidomics.
Why it fascinates you?
The thrill of discovery provides plenty of passion to get out of bed each day to chase down structure activity relationships.
Research Focus
Fast lipid analysis technique (FLAT)
Interrogating the metabolic differences found between environments that support improved prognosis and poor prognosis.
MicroPOTs
With our collaborators, who developed nanoPOTS, we’ve developed a novel technique called microPOTS (Weke et al. JPR 2021) that allows proteomic analysis on small sample sizes (~ 200 cells) and can identify over 1500 proteins. We are now adapting a related technique, autoPOTS, to include metabolomic analysis as well.
Mass spectrometry imaging (MSI)
We are developing MSI techniques to image lipids from spirochetes (e.g. Treponema and Leptospira) from in vivo sources. We will use MSI to probe differences in structure between in vitro and in vivo produced microbial lipids, the differences in which can drastically change the structure activity relationship.
